Innovations and Solutions of Intact Biopsy Solid-state NMR Spectroscopy at 1200 MHz towards 1535 MHz Ultrahigh Magnetic Fields

Abstract

Recent advances and progress in high resolution magic angle spinning (HR-MAS) ultrahigh magnetic field in vivo NMR spectroscopy has evidenced unique opportunity in   deep learning of intact human brain glioblastoma  tumors,  astrocytoma,  Schwannoma, metastases  from adrenocarcinoma  and breast cancer. Further, advancements  are in progress at magnetic resonance microimaging and spectroscopy with high resolution and sensitivity of human tissues. New superconducting magnets at a magnetic field strengths (magnetic induction) of 28.2 T or 1200 MHz – 35 T o r 1 5 0 0 M H z proton resonance frequency have become commercially available. First time, new possibilities are highlighted on ultrahigh-field 36 T MR microimaging and NMR spectra of intact tissue proteins responsible as molecular machines, membrane proteins and energy molecules. Present paper represents the scope of emerging HR- MAS NMR spectroscopy new possibilities of deep learning in the light of fundamental MAS- MRI/NMR imaging, biochemical and physiological nanophysics 1D-HSQC principles to explore new achievements in acquiring pre-clinical and functional NMR visible over 37 metabolites to classify and grade the human brain glioblastoma tumors and excised human biopsy tissues from lung, breast, colon and gastrointestinal systems. The known carbon-13- proton HR- MAS NMR experiments are illustrated for aliphatic side-chain resonances in cancer tissue proteins in evaluation and possible prediction of human cancer disease physiochemistry.