Study of Anticholinergic Effect of Moringa Pterygosperma Leaves Extract on Intestinal Smooth Muscle in comparison with Atropine

Abstract

Anticholinergics are the agent that inhibits neurotransmitter acetyl choline in the parasympathetic outflow. Moringa pterygosperma is a commonly available plant that has been used commercially for its anticholinergic property. It also possesses antioxidant, anti-diabetic, anti-inflammatory, anti-ulcer and hypocholesteromic properties. The aim of the study is to assess the Anticholinergic activity of ethanolic extract of Moringa pterygosperma in comparison with Atropine using in vitro experimental models. Ethanolic extract was prepared by soxhlation method. For in vitro study, isolated chick ileum was used. Chick ileum was suspended in an organ bath containing Tyrode solution at 37◦C with adequate oxygen supply. Effect of the Moringa pterygosperma extract was studied on ileum contractions induced by Acetylcholine and compared with that of atropine. Mean percentage response was calculated for ethanolic extract of Moringa pterygosperma and atropine. Ethanolic extract of Moringa pterygosperma and atropine inhibited ileum contraction induced by Acetylcholine. From this study it was concluded that Moringa pterygosperma extract have significant anticholinergic activity compared to atropine. These findings therefore raise hope for the development of a new anticholinergic drug with few side effects that may be useful in the treatment of conditions like diarrhoea, asthma, incontinence, peptic ulcer, and muscle spasms.