CHICKEN ILEUM: A PREFERRED MODEL FOR PHARMACOLOGICAL AND PHYSIOLOGICAL STUDIES: A REVIEW

Abstract

The in-vivo approach to animal testing involves conducting experiments on non-human animals. Non-human primates, mice, rats, rabbits, guinea pigs, and hamsters are often used laboratory animals. In silico computer models and in vitro cell culture methods are substitutes for in vivo testing.. The existing restrictions on laboratory animal experimentation necessitate the exploration of alternative biological tissues for testing purposes. In undergraduate and graduate pharmacology studies, bioassays are essential. They use a variety of isolated tissues, including the rectus of frogs, the colon of rats, the ileum of chickens, and the ileum of guinea pigs. To address the issue, the user conducted a bioassay experiment using chicken ileum obtained from a slaughterhouse, which is a more readily available and humane option. The application of Tyrode solution yielded a higher response magnitude and enhanced stability in the bioassay experiment when compared to Ringer Locke solution. The interpolation method of bioassay was used to assess the concentration of acetylcholine, which was found to be 82.17% accurate compared to the actual concentration. A more suitable alternative for performing isolated tissue experiments on experimental animals in accordance with CPCSEA Guidelines had been suggested. The application of isolated chicken ileum as a substitute for laboratory animals in bioassay experiments holds considerable promise for minimizing animal sacrifice and fostering more ethical research practices. This methodology may enhance experimental procedures and tissue utilization, potentially resulting in the advancement of more efficient and effective research techniques.