Nitroimidazole Triggers the DNA Synthesis in Kupffer Cells Towards Liver Regeneration and Restoration

Abstract

To evaluate the effect of nitroimidazole on isolated human Kupffer cells in cultures by using DNA synthesis activity as Kupffer cell biomarker with evidence of DNA synthesis regulatory behavior. Hypothesis: Kupffer cell DNA synthesis activity may be a biomarker of antiamoebic cytotoxicity evaluation of nitroimidazole. Methods and Materials: The selected liver biopsies from patients (ongoing amebic liver abscess research project) were processed for isolation and fractionation of Kupffer cells.  Three groups of Kupffer cells were: untreated control cells (group I); cells from liver abscess infected liver biopsy (group II); and cells from nitroimidazole treated liver abscess biopsy (group III).  The DNA synthesis activities in cells were measured. Results: The DNA synthesis activities showed inhibited DNA synthesis activity significantly (r²=0.5; p value 0.001) by nitroimidazole. Discussion: The DNA synthesis activity is progesterone hormonal dependent and regulated at gene level.  In Kupffer cells, gene expression control by new DNA synthesis is significant after nitroimidazole treatment in enhancing phagocytosis in humans. The nitroimidazole effect was positive in Kupffer cells towards liver regeneration.  The effect of nitroimidazole was less likely to influence the gene expression in damaged amoebic cells. Conclusion: The nitroimidazole directly affects the human Kupffer cell DNA synthesis as antiamoebic activity to secure the hepatocyte regeneration. The DNA synthesis activity in Kuppfer cells can be used as liver regeneration biomarker. The DNA synthesis is significant in defining the liver regeneration