Comparative Gastro Protective Effects of Monofloral Honey, Misoprostol and Monofloral Honey Misoprostol Combination against Indomethacin Induced Gastric Ulcer in Albino Rats

Abstract

Background and objectives: Oxidative damage, imbalanced synthesis of nitric oxide, and local recruitment of mononuclear phagocytes are the major mediators of indomethacin-induced gastric ulcers. Monofloral honey (MFH) is a powerful flavonoid antioxidant that prevents neutrophil infiltration and reduces nitric oxide generation in the stomach. Therefore, this research level to investigate the gastroprotective effects of MFH opposed indomethacin-induced gastric injury. Materials and Methods: This study was conducted at the Department of Pharmacology, Dhaka Medical College, Dhaka. Albino rats were assigned to six groups (groups 1 to 4) and each group consisted of six rats. Gr1 received a single oral administration of indomethacin (30 mg/kg body weight) and Gr2, 3, and 4 were treated with assigned doses of misoprostol, MFH , and MFH + misoprostol, respectively, for 7 days prior to ulcer induction. The effects of experimental agents were evaluated by Ulcer Score, Ulcer Index (UI), Percent Protection Ratio (PPR). The effects of MFH, misoprostol, and a mixture of MFH and misoprostol were compared to high-dose indomethacin-induced gastric ulcer in rats. Results: UI was significantly (<0.001) lower in misoprostol (1.33±0.81), MFH (1.50±0.54) and MFH + misoprostol (0.67±0.81) treated group compared to indomethacin treated group (2.83±0.75). The PPR of misoprostol, MFH, and MFH + misoprostol was 53.60%, 46.90%, 76.32%, respectively. Conclusions: These results suggest that MFH protects indomethacin-induced gastric ulcers by reducing free radical production, neutrophil infiltration, and restoration of physiological tissue nitrate levels independent of gastric acidity.