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Development and Validation of HPTLC Method for Simultaneous Estimation of Azelnidipine and Telmisartan

Heta Kachhiya, Jinal Tandel, Isha Rupchandani, Adit Velani, Suchi Patel, Krupa Patel, Nirali Zala

Abstract


HPTLC method for simultaneous estimation of Azelnidipine and Telmisartan in bulk and their pharmaceutical dosage form was developed and validated. Pre coated silica gel G60-F254 aluminium sheet as a stationary phase and Toluene: Acetonitrile: Formic acid (5:4.5:0.5 % V/V/V) as mobile phase was optimized to develop chromatograph.  The method shown linearity in the range of 200-700 ng/ band   for   Azelnidipine and   1000-3500 ng/band for Telmisartan with R2 0.9961 and 0.9971 for Azelnidipine and Telmisartan respectively. %Recovery of 100.1 to 103.8% for Azelnidipine and 99.59 to 100.8% for Telmisartan was achieved by applying standard spiking method of accuracy study. LOD and LOQ values were 24.71 ng/band and 74.90 ng/bank for Azelnidipine. LOD and LOQ values were 175.04 ng/band and 530.44 ng/bank for Telmisartan. A sensitive, accurate, precise, rapid, cost effective and less time-consuming method is repeatable and selective for the analysis of Azelnidipine and Telmisartan without having any interference from excipients.


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References


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