Analytical Method Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Mefenamic acid and Tizanidine HCl

Abstract

High performance liquid chromatography (HPLC) method has been developed and validated for the estimation of Mefenamic acid and Tizanidine tablets. A simple, rapid, accurate and precise HPLC method was developed for quantification of Mefenamic acid and Tizanidine HCl. The developed method used the mixture of Acetonitrile and Water (90:10, v/v) as mobile phase at a flow rate of 1.0 mL/min, Detection was carried out at 250 nm. The linearity for Mefenamic acid of the proposed method was found in the concentration range of 125-625 ppm with regression coefficient (R²) of 0.998 and the linearity for Tizanidine of the proposed method was found in the concentration range of 0.75-2.5 ppm with regression coefficient (R²) of 0.999. LOD and LOQ for Mefenamic acid were found to be 0.1847µg/ml and 0.5598µg/ml and LOD and LOQ for Tizanidine were found to be 0.0151µg/ml and 0.0458 µg/ml, respectively. The % recovery of the suggested technique for Mefenamic acid was set up to be 99.2 %-99.7% and for Tizanidine was found to be 99.5 %-100.3%. The method was found to be precise and robust. The developed HPLC method was successfully applied for quantification of Mefenamic acid and Tizanidine in marketed tablet.