Open Access
Subscription Access
A Review on UHPLC Instrumentation with Advancements
Abstract
The Ultra High Performance Liquid Chromatography (UHPLC) is the technique of liquid chromatography which improve speed, resolution and sensitivity. The instrumentation involves the advance requirements that helps the quick response of the peaks. The advances in instrumentation improve the fast separation with the help of UHPLC column involved in the front line of liquid chromatography column developed by giving higher quality chromatographic data in less time. The advancement in column like Columns packed with 2.6-2.7-μm SCPs, Columns packed with sub-2-μm SCPs and Charged surface-particle technology improving the good quantification of the separation of products. This technique provides unique end-to-end solutions for all industries and has found application in various fields such as pharmaceutical analysis, food analysis, environmental analysis, determination of pesticides in groundwater, rapid does formulation analysis and others.
References
Walter TH, Andrews RW. Recent innovations in UHPLC columns and instrumentation. Trends Anal Chem 63:14-20(2014).
Singh S, Prasad L, Kumar V, et al. Ultra Performance Liquid Chromatography:high throughput analysis over high performance liquid chromatography: Int J Eng Allied Sci2:19-25(2016).
Samatha Y, Srividya A, Ajitha A, et al. Ultra Performance Liquid Chromatography. World J Pharma Pharma Sci 8 (2015):356-367.
Wales, Thomas E, et al. "High-speed and high-resolution UPLC separation at zerodegrees Celsius." Analytical chemistry 2008; 80.17: 6815-6820.
UPLC columns and consumables, https://www.waters.com//UPLCColumn 2014.
Narwate BM, Ghule PJ, Ghule AV, et al. Ultra performance liquid chromatography: A new resolution in chromatography. Int J Pharm Drug Anal 2(2015):25–34.
Marca GL, Giocaliere E, Villanelli F, et al. Development of an UPLC– MS/MS method for the determination of antibiotic ertapenem on dried blood spots. J Pharm Biomed Anal 61(2012):108–113.
Chawla G, Ranjan C. Principle, instrumentation and applications of UPLC: A novel technique of liquid chromatography. Open Chem J 3(2016):1-16.
Acquity UPLC columns by waters, https://www.waters.com 2008.
Prathap, GM, Nishat A. Ultra Performance Liquid Chromatography: A Chromatography TECHNIQUE. Int. J. Pharmacy, 2013,3(1)00000:251-260.
Lars Y, Honore HS. On-line turbulent-flow chromatography high performance liquid chromatography-mass spectrometry for fast sample preparation and quantitation. J. Chromatogr 2003, A 1020:59-67.
Broske AD. Agilent technologies application note, 2004. Available at: https://www.adilent.com/cs/library/primers/Public/5991- 3326EN_SPHB.pdf
Fountain KJ, Neue UD, Grumbach ES, et al. Effects of extra-columns band spreading, liquid chromatography system operating pressure and column temperature on the performance of sub-2-μm porous particles. J, Chromatogr, A1216 (2009): 5979-5988.
Fountain KJ, Iraneta PC. Instrumentation and column for UHPLC separation in: Guillarme D, Veuthey JL. (Editors), UHPLC in Life Science, No.16, RSC Chromatography monograph, Cambridge, UK, 2012. Pg:29-66.
Gritti F, Sanchez CA, Farkas T, et al. Achieving the full performance of high efficient columns by optimizing conventional benchmark high performance liquid chromatography instruments. J. Chromatogr, A1216 (2009):3000-3012.
Claessens HA, Cramers CA, Kuyken MAJ. Estimation of the band broadening contribution of HPLC equipment to column elution profiles Chromatographia 23 (1987):189-194.
Gritti F, Guiochan G. Optimization of the thermal environment of columns packed with very fine particle J. Chromatogr, A1216 (2009):1353-1362.
Bobaly B, Guillarme D, Fekete S. Systemic comparison of a new generation of columns packed with sub-2-μm superficially porous particles, J. Sep. Sci.37(2014): 189-197.
De Villiers A, Lynen F, Sandra P. Effect of analyte properties on the kinetic performance of liquid chromatographic separation, J. Chromatogr, A1216(2009): 3431-3442.
Foster MD, Amold MA, Nichols JA, et al. Performance of experimental sample injector for high performance liquid chromatography micro columns, J. Chromatogr, A869(2000):231-234.
Waters ACQUITY UPLC TUV Detector Specification, 2014.
Spaggiari D, Fekete S, Eugster PJ, et al. Contributation of various types of liquid chromatography mass spectrometry instruments to band broadening in fast analysis. J. Chromatogr, A1310(2013):45-55.
Kuss HJ, Integration errors, in: Kuss, Kromidas S (Editors). Quantification in LC and GC, Wiley VCH, Weinheim, 2009. Pg:37-43.
Roge AB, Firke SN, Dhane RM, et al. Novel achievement of HPLC: UPLC. Int J Pharm Tech Res 3(2011):1423-1429.
Zhang, Bin, Xiaofeng Li, et al. "Advances in HPLC detection towards universal detection." Analytical and Bioanalytical Chemistry; 390(1):299-301.
Principles and application of LC-MS/MS for the quantitative bioanalysis of analytes in various biological sample. In: tandem mass spectrometry application and peinciples:276-289(2012).
Chawla G, Ranjan C. Principle, instrumentation and application of UPLC:Anovel technique of liquid chromatography. Open Chem J 3(2016):1-16.
Acquity UPLC column by water. https://www.waters.com 2008.
Mazzen JR, Neue UD, Kele M, et al. A new separation technique takes advantage of sub-2-μm porous particles. Anal. Chem. 77 (2005):460A-467A.
Wyndham KD, O’Gara JE, Walter TH, et.al. Characterization and evaluation of HPLC stationary phases based on ethyl-bridge hybrid organic/inorganic particles. Anal. Chem. 75 (2003):6781-6788.
McCalley DV, Shell particles and UHPLC technologies for fast analysis of polar compounds in the HILIC mode, in: Guillarme D, Veuthey JL (Editors). UHPLC in Life Science, No. 16, RSC Chromatography monographs, Cambridge,UK, 2012. Pg:164-185.
Kotoni D, Ciogli A, Molinaro C, et.al. Introducing enantio selective Ultra High Pressure Liquid Chromatography(eUHPLC): theoretical inspection and ultra fast separation on a new sub-2-μm Whelk-01 stationary phase. Anal. Chem. 84 (2012):6805-6813.
Rea JC, Wang YJ, Zhang T. UHPLC for characterization of protein therapeutics, in: Q.A. Xu (Editor), Ultra High Pressure Liquid Chromatography and Its Application, Jhone Wiley &Sons, Hoboken, NJ, 2013. Pg:235-252.
Bouyier ESP, Koza SM. Advances in size-exclusion separation of proteins and polymer by UHPLC. Trends Anal. Chem. 36 (2014):85-94.
Neue UD, Kele M, Bunner B, et.al. Ultra High Pressure Liquid Chromatography and application. Adv. Chromatogr,48 (2009):99-104.
Guiochan G, Gritti F. Shell particles, trials, tribulation and triumphs. J.Chromatogr, A 1218 (2011):1915-1938.
Gritti F, Guiochon G. Repeatability of the efficiency of columns packed with sub-2-μm core-shell particles: part 3, 2.7μm Poroshell 120 EC-C18 particles in 4.6mm and 2.1mm* 100mm columns formets. J. Chromatogr, A 1252 (2012):56-66.
Gritti F, Guiochon G. Mass transfer resistance in narrow bore columns packed with 1.7 μm particles in high performance liquid chromatography. J. Chromatogr. A 1217 (2010):5069-5083.
Omamogho JO, Glennon JD. Comparison between the efficiencies of sub-2-μm C18 particle packed in narrow bore columns. Anal. Chem. 83(2011):1547-1556.
Omamogho JO, Hanrahan JP, Tobin J, et al. Structural variation of solid core and thickness of porous shell of 1.7μm core-shell silica particles on chromatographic performance: narrow bore columns. J. Chromatogr. A 1218 (2011):1942-1953.
Sanchez AC, Friedlander G, Fekete S, et.al. Pushing the performance limits of reversed phase ultra high performance liquid chromatography with 1.37μm core-shell particles. J. Chromatogr. A 1311 (2013):90-97.
Gritti F, Guiochon G. Rapid development of core-shell column technology: accurate measurements of the intrinsic column efficiency of narrow-bore column packed with 4.6 down to 1.3 μm superficially porous particles. J. Chromatogr.A 1333 (2014):60-69.
Fekete S, Guillarme D. Kinetics evaluation of new generation of column packed with 1.3 μm core-shell particles. J. Chromatogr. A 1308 (2013):104-113.
De Villiers A, Lynen F, Sandra P. Effect of analyte properties on the kinetics performance liquid chromatographic separations. J. Chromatogr. A 1216 (2009):3431-3442.
Neue UD, Kele M, Bunner B, et al. Ultra-Performance liquid chromatography technology and applications. Adv.Chromatogr. 48 (2009):99–104.
Rea JC, Wang YJ, Zhang T. UHPLC for characterization of protein therapeutics Ultra- High Performance Liquid Chromatography and Its Applications. NJ, 2013. Pg:235-252.
Wu, Naijun and Richard Thompson. "Fast and efficient separations using reversed phase liquid chromatography." Journal of Liquid Chromatography 2006;29(7-8):949-988.
Nguyen, Dao TT, Sandra P, et al. "High throughput liquid chromatography with sub-2μm particles at high pressure and high temperature." Journal of Chromatography A 2007; 1167.1:76-84.
Swartz, Michael E. "UPLCTM: an introduction and review." Journal of Liquid Chromatography & Related Technologies 2005; 28(7-8):1253-1263.
Wren, Stephen AC and Pierre Tchelitcheff. "Use of ultra-performance liquid chromatography in pharmaceutical development." Journal of Chromatography A 2006 ;1119(1):140-146.
Refbacks
- There are currently no refbacks.