Comparison of the fungi's radial growth tests on potato dextrose agar and prepared cactus pear cladode agar

Abstract

The purpose of this study is to determine whether cactus pear (Opuntia ficus-indica L.) cladode extracts can be used to make fungal culture media. R.stoloinfer ATCC 1052, P.digitatum ATCC 2560, A.niger ATCC 1232, T.aueoviride ATCC 2156, and S.cerevisea ATCC 234) were tested for nine days at 25°C on cactus pear cladode agar w/v (4:200, 4:250, 4:300, and 4:350). The fungal colony's radial growth (RG R. stoloinfer grew quickly and covered the entire petriplate diameter in 24 hours, surpassing A. niger, P. digitatum, and T. aueoviride, which recorded higher RG at TS2 (30.250.54, 15.850.59, and 11.580.63). P.digitatum and T.aueoviride had significantly (p0.05) higher RG at TS1 than PDA (18.830.17 and 10.830.52), respectively. However, A. niger produced RG at TS1 that was comparable to that of PDA (34.08  0.76). At TS1 (16.330.57), S.cerevisea produced less RG than PDA (20.420.88). In conclusion, a higher RG was observed on TS1, indicating that the medium has a significant potential to replace the PDA. However, additional growth performance evaluation parameters are necessary to determine whether the medium is suitable for commercial use.