Evaluation of Leaf Extract of Amorphophallus Paeoniifolius for Antioxidant potential, TPC, TFC and HPTLC Fingerprinting

Abstract

Amorphophallus paeoniifolius plant belongs to Araceae family, commonly known as Jimmikant, White spot arum, jungli suran, etc. The plant has been investigated and found to have many medicinal properties. Leaves of Amorphophallus paeoniifolius are having medicinal values such as anti-diarrhoeal, anti-bacterial properties. Also, recently 13 flavonoids have been isolated from the leaves of Amorphophallus paeoniifolius. The present research evaluates the estimation of methanolic extract of leaves of plant for Total Phenolic Content and Total Flavonoid Content. Total phenolic content in methanolic extract of leaf was found to be 21.43 mg/g GAE, along with this total flavonoid content was found to be 11.96 mg/g QE. High performance thin layer chromatography (HPTLC) profile of the methanolic leaf extract was also performed to compare with standard biomarkers such as Resveratrol, Lupeol and β-sitosterol using silica gel G60 F₂₅₄ as stationary phase and Benzene: Ethyl acetate (9:1 v/v) as mobile phase. The quantitative study was carried out using HPTLC which demonstrates that methanolic extract of leaf contains Resveratrol, Lupeol and β-sitosterol with R_f values of 0.03, 0.53 and 0.36 respectively.