Open Access
Subscription Access
Cardiomyocytes Modulating Effects Of Annona Muricata (Soursop) Leaf Extract On Testosterone - Induced Benign Prostatic Hyperplasia In Male Wistar Rats
Abstract
This present study aimed to determined Cardiomyoctes modulating effects of annona muricata (soursop) leaf extract on testosterone – induced benign prostatic hyperplasia. A total of 78 adult male rats obtained from the laboratory animal production unit of the Department of Physiology, Abia State University, Uturu were used for the different segments of the study. Eighteen of the rats were used for acute toxicity/lethal dose evaluation of the extract while the remaining sixty (60) were used for the testosterone-induced hyperplasia study. Prostate hyperplasia was induced in the rats via daily sub-cutaneous administration of testosterone propionate (5 mg/kg body weight) for 28 days. Sixty rats used for this segment of the study were assigned to 6 groups of 10 rats and treated as follows: Group 1, Normal control, no treatment. Group 2 Testosterone propionate (5 mg/kg) only and was the BPH control. Group 3 ,Testosterone propionate (5 mg/kg bw) + Finesteride (3 mg/kg body weight).Group 4, Testosterone propionate (5 mg/kg bw) + Annona Muricata Leaf extract (300 mg/kg body weight). Group 5, Testosterone propionate (5 mg/kg bw) + Annona Muricata Leaf extract (600 mg/kg body weight).Group 6, Annona Muricata Leaf extract (600 mg/kg bw) only. At the end of treatment, animals were sacrificed, Organs such as the heart were harvested, weighed and preserved in labelled sample bottles containing 10% formaldehyde solution for histopathological studies of the heart. Data obtained were suggested to Statistical Package for Social Sciences (SPSS, Version 20.0, IBM SPSS Inc, Chicago, IL) was used for data analysis. Level of significance was calculated by One Way Analysis of Variance (ANOVA). Data were analyzed using Duncan Multiple Range Test and complemented with Student’s t test for post-hoc test for comparisons of the means of the various doses and fractions. P- values less than 0.05 were considered statistically significantly different between the test and control groups as well as among test groups for measured value. From this present study, histopathological evaluations of the heart, consistently revealed preserved heart tissue architecture in treated animals with Annona muricata leaf extract highlighting the extract's tissue-protective properties in benign prostatic hyperplasia.
References
Al-Medhtiy, M. H., Jabbar, A. A., Shareef, S. H., Ibrahim, I. A. A., Alzahrani, A. R., and Abdulla, M. A. (2022). Histopathological evaluation of Annona muricata in TAA-induced liver injury in rats. Processes, 10(8): 1613.
Champy, P., Melot, A., Guérineau Eng, V., Gleye, C., Fall, D., Höglinger, G. U., ...and Hocquemiller, R. (2005). Quantification of acetogenins in Annona muricata linked to atypical parkinsonism in Guadeloupe. Movement disorders: official journal of the Movement Disorder Society, 20(12): 1629-1633.
Ezeh, A. K., Nwankwo, A., Ifemenam, K. E., Nwozor, C. M., Ucheonye, B. N., and Okwummuo, J. I. (2024). Effect of ethanolic stem bark extract of Annona muricata (soursop) on reproductive hormones and ovary in female Wistar rats. World Journal of Biology Pharmacy and Health Sciences, 18(2): 280-288.
Fofana, S., Keita, A., Balde, S., Ziyaev, R., and Aripova, S. F. (2012). Alkaloids from leaves of Annona muricata. Chemistry of Natural Compounds, 48: 714-714.
Gavamukulya, Y., Wamunyokoli, F., and El-Shemy, H. A. (2017). Annona muricata: Is the natural therapy to most disease conditions including cancer growing in our backyard? A systematic review of its research history and future prospects. Asian Pacific journal of tropical medicine, 10(9): 835-848.
Ibukun, O. E., Ogunlade, L. O., and Oladipo, G. O. (2023). Annona muricata (Soursop) Mitigated Testicular Toxicity and Prostatic Impairment in Testosterone-Propionate-Induced BPH in Male Rats. Achievers Journal of Scientific Research (AJOSR), 5(2).
Manrique-de-la-Cuba, M. F., Gamero-Begazo, P., Valencia, D. E., Barazorda-Ccahuana, H. L., and Gómez, B. (2019). Theoretical study of the antioxidant capacity of the flavonoids present in the Annona muricata (Soursop) leaves. Journal of Molecular Modeling, 25: 1-13.
Moghadamtousi, S. Z., Fadaeinasab, M., Nikzad, S., Mohan, G., Ali, H. M., and Kadir, H. A. (2015). Annona muricata (Annonaceae): a review of its traditional uses, isolated acetogenins and biological activities. International journal of molecular sciences, 16(7): 15625-15658.
Mutakin, M., Fauziati, R., Fadhilah, F. N., Zuhrotun, A., Amalia, R., and Hadisaputri, Y. E. (2022). Pharmacological activities of soursop (Annona muricata Lin.). Molecules, 27(4): 1201.
Nolasco-González, Y., Chacón-López, M. A., Ortiz-Basurto, R. I., Aguilera-Aguirre, S., González-Aguilar, G. A., Rodríguez-Aguayo, C., ...and Montalvo-González, E. (2022). Annona muricata leaves as a source of bioactive compounds: Extraction and quantification using ultrasound. Horticulturae, 8(7): 560.
Olude, O. M., Omoregie, F. A., & Chukwu, O. J. (2023). Protective Effect of Ethanol Extract of Annona Muricata Leaves on Hematological Profile, Histology and Oxidative Stress in 1, 2-Dimethylhydrazine-induced Colorectal Carcinogenesis in Rats. Journal of Applied Sciences and Environmental Management, 27(2): 257-265.
Padma, P., Chansouria, J. P. N., and Khosa, R. L. (1999). Hepatoprotective activity of Annona muricata Linn. and Polyalthia cerasoides bedd. Ancient Science of life, 19(1and2): 7-10.
Prasad, S. K., Pradeep, S., Shimavallu, C., Kollur, S. P., Syed, A., Marraiki, N., ...and Glossman-Mitnik, D. (2021). Evaluation of Annona muricata acetogenins as potential anti-SARS-CoV-2 agents through computational approaches. Frontiers in chemistry, 8: 624716.
Refbacks
- There are currently no refbacks.