Open Access Open Access  Restricted Access Subscription Access

Comparing the Radial Growth Assay of Fungal Species on Potato Dextrose Agar and Prepared Cactus Pear Cladode Agar

Rakhi Arora

Abstract


This study evaluates the potential of cactus pear (Opuntia ficus-indica L.) cladode extracts for preparing fungal culture media. The growth of five fungal species—Rhizopus stolonifer (ATCC 1052), Penicillium digitatum (ATCC 2560), Aspergillus niger (ATCC 1232), Trichoderma aureoviride (ATCC 2156), and Saccharomyces cerevisiae (ATCC 234)—was assessed on cactus pear cladode agar (CPCA) at different concentrations (4:200, 4:250, 4:300, and 4:350 w/v) over nine days at 25°C. Radial growth (RG) was measured every 72 hours and compared to that on potato dextrose agar (PDA). R. stolonifer exhibited the fastest growth, fully covering the petri dish by 24 hours. The highest RG was observed in the 4:200 concentration (TS1), with A. niger, P. digitatum, and T. aureoviride also showing significant growth compared to PDA. However, S. cerevisiae demonstrated lower RG on TS1 compared to PDA. The results indicate that cactus pear cladode extracts have substantial potential as a substitute for PDA in fungal culture media, although further evaluation of other growth performance parameters is necessary for commercial applicability.


Full Text:

PDF

References


Adesemoye, A., & Adedire, C. (2005). Use of cereals as basal medium for the formulation of alternative culture media for fungi. World Journal of Microbiology & Biotechnology, 21, 329-336. https://doi.org/10.1007/s11274-004-2211-7

Anbu, S., Padma, J., Punithavalli, K., & Saranraj, P. (2017). Fruit peel waste as a novel media for the growth of economically important fungi. Journal of Pharmacognosy and Phytochemistry, 6(6), 426-428.

AOAC. (1984). Official methods of analysis. Association of Official Analytical Chemists, Arlington, VA.

Bharti, N. M., Sandip, P., Amit, K., & Sharma, P. C. (2013). Novel preparations of culture media from plant materials for cultivation of Candida albicans. International Journal of Pharmaceutical Sciences Review and Research, 20(1), 128-133.

FDA. (2003). Food sampling and preparation of sample homogenate. In Bacteriological Analytical Manual Online (8th ed.). Food and Drug Administration (FDA), USA.

Hanan, H., Youssef, M. A., Hamza, M. F., Elhussein, F., Mourad, M. Y., Saleh, M. S., Sarhan, R. M., Suker, A. A., Eltahlawy, R. A., Nemr, M., El-Tahan, S. R., & Nabil, A. H. (2016). Plant-based culture media: Efficiently supporting culturing rhizobacteria and correctly mirroring their in-situ diversity. Journal of Advanced Research, 7, 305-316. https://doi.org/10.1016/j.jare.2015.11.004

HPA. (2004). Preparation of samples and decimal dilutions. National Standard Method D1, Issue No. 2. Health Protection Agency (HPA), UK.

Kunyanga, C. N., Vellingiri, V., & Imungi, K. J. (2014). Nutritional quality, phytochemical composition and health protective effects of an under-utilized prickly cactus fruit (Opuntia stricta Haw.) collected from Kenya. African Journal of Food, Agriculture, Nutrition and Development, 14(7).

Lee, E. H., Kim, H. J., Song, Y. S., Jin, C., & Lee, K. T. (2003). Constituents of the stems and fruits of Opuntia ficus-indica var. saboten. Archives of Pharmacal Research, 26, 1018-1023. https://doi.org/10.1007/BF02976371

Martinez-Rodríguez, M. C., Rincón, F., Periagon-Castro, M. J., Ros-Berruezo, G. F., & López, G. (1993). Dietary fiber components and physiological effects. Spanish Journal of Science and Food Technology, 33, 229-246.


Refbacks

  • There are currently no refbacks.