Simultaneous Estimation of Amlodipine and Valsartan by Using Reverse Phase High Performance Liquid Chromatography in Bulk and Marketed Formulation

Abstract

A new method was advanced and authorized for simultaneous estimation of Amlodipine and Valsartan using RP-HPLC. The maximum absorbance for both drugs was observed at 225nm. The Altima C18 column was chosen for the study due to its ability to produce sharp, well-resolved peaks for both amlodipine and valsartan. Method was developed using mobile phase methanol: phosphate buffer (pH 4.5) Acetonitrile in the ratio (50:25:25) with injection volume 10 μl, flow rate 1.0ml/min and run time was established to ensure adequate separation, with amlodipine and valsartan eluting at approximately 2.102 and 3.537 minutes ±0.02, respectively, while keeping the analysis time efficient. The method was found to be linear over the concentration ranges of 5–25 µg/mL for amlodipine and 12.5–62.5 µg/mL for valsartan, with good correlation coefficients. The percent recovery was found to be within the acceptable range of 98.0% to 102.0%, indicating accuracy and minimal interference. The method demonstrated good precision, with %RSD values well within the acceptable limit of 2%. The results obtained through the RP-HPLC method were reliable and consistent, as presented in the respective tables. Furthermore, this method proved to be more sensitive, accurate, and precise compared to spectrophotometric methods.