Assessment of Electrolyte Concentrations of Ethanoic Extract of Ripe and unripe Banana fruits in salt loaded Alcoholic and indomethacin induced Ulcerative Rats

Abstract

The increasing popularity and consumption of herbal remedies all over the globe has also brought many clinical challenges. The OTC nature of these herbal remedies and their ease of availability largely facilitate the population upon its utilization in different disease states along with conventional drugs and supplements which not only raise chances of adverse effects but herb-drug and herb-supplement interactions. Due to wide consumption of different parts of banana fruits for different therapeutic purposes, this study aims to examine the Electrolyte Concentrations of Ethanoic Extract of Ripe and unripe Banana fruits in salt loaded, Alcoholic and indomethacin induced Ulcerative Rats. Eighty male wistar rats (weighing between 120-140g were grouped into 16 standard study groups of 5 animals each. Group A: Control group this group received food and water only. Group Bi: Ulcer induced by indomethacin (NSAID) without treatment Group Bii: Ulcer (Alcohol) without treatment Group C: Received Sodium chloride orally for 2 weeks without treatment, Group Di: Received Sodium chloride orally all through the study (NaCl)+ Ulcer (NSAID) without treatment Group Dii: Received Sodium chloride orally all through the study (NaCl)+ Ulcer (Alcohol ) without treatment, each of the group were treated with unripe banana extract and ripe banana extract, all animals were sacrificed after exposure to chloroform. Blood was collected through cardiac puncture, placed in a lithium heparin sample bottles. Blood samples was spun in a bucket centrifuge at 2500 RPM (rounds per minute) for 10 minutes after which plasma was collected and stored frozen in plain sample bottles and was analyzed for electrolyte concentrations (Chloride, Bicarbonate , Sodium and Potassium levels) by spectrophotometric method. Data obtained were analyzed as mean ± standard deviation using one way analysis of variance (ANOVA. Level of significance between groups was taken as p<0.05. There was no significant effect on Bicarbonate, Sodium and Potassium levels. The significant decrease in the Chloride levels of the group that received Omeprazole before ulcer induction using Indomethacin when compared to the control group as observed in this study is suggesting that either Omeprazole or Indomethacin does not support procedures that leads to retention of water by the kidney or omeprazole blocked the indomethacin water retention actions in the kidney. There was no significant difference between stomach electrolytes levels of the following experiment groups (NaCl+Unripped Banana) and (NaCl+Ripped Banana), NaCl+Unripped Banana+NSAID) and (NaCl+c+ALCOHOL), NaCl+Ripped Banana+NSAID) and (NaCl+Ripped Banana+ALCOHOL). From the following observations made in this present study it could be suggested that both ripe and unripe banana have the same effect on the electrolyte levels of the body, it could also be suggested that both Nacl, NSAID and Alcoholic actions on the body electrolytes could be protected by both ripped and unripe banana.