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Effect of Clomiphene Citrate on The Kidney of Adult Female Wister Rats with Regular Estrous Cycle

Olugbenga, M. A., Adelana Damilola, Ibhadode Adesua, Ohiwerei F. O., Anslem O. Q, Otaye, O. M, Ohiwerei. W. O.

Abstract


The aim of this study is to evaluate and assess the Effect of Clomiphene citrate on kidney of adult female wistar rats with regular estrous cycle. A total of sixteen (16) 10 weeks old healthy female Wistar rats were purchased for the purpose of this study. Group 1(Control) (12 weeks old female rats; control): The animals in this group were not exposed to any experimental agent/drug. Group 2 (12 weeks old female rats; test): The animals in this group were exposed to the test drug (Clomiphene citrate  1mg) for 11 consecutive days.  Group 3 (12 weeks old female rats; test): The animals in this group were /exposed to the test drug (Clomiphene citrate  2mg) for 11 consecutive days. Group 4 (12 weeks old female rats; test): The animals in this group were exposed to the test drug (Clomiphene citrate  5 mg) for 11 consecutive days. The test group rats were treated with Clomiphene Citate (30 mg body weight daily dissolved in required quantity of normal saline. Vaginal smear method to determent the estrous cycle of the rats was as documented.Vaginal smears were obtained from all rats to determine whether they had regular cycles for three cycles before drug administration. At the end of the 11th day of drug administration, vagina smear was carried out and the rats were sacrificed by cervical dislocation.  Histological processing was done following the standard histological method. The result reveals that effect of different doses of Clomiphene citrate on kidney weight for regular estrous cycle. There were  non significant (p>0.05) changes in organ weight from 0.80±0.25g for control to 0.97±0.07, 0.93±0.09 and 1.03±0.19g for group B, group C and group D respectively. Histology of the kidney showed degenerated glomerulus with demote spaces, mild degeneration of the glomerulus and mild leukocytic infiltration in some groups. It  is  concluded  from  the  result  of the  present  work  that  Clomiphene  citrate  induced  kidney injury  via its oxidative stress.


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