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The Histological Effects of Chloroquine on the Kidney in Male wistar Rats

Okoronkwo C.A., Edebiri O.E., Ogedegbe A., Okekpa U.O., Omende K., Orunta E.D, Okike P.I.

Abstract


The aim of this study is to determine the histological effects of chloroquine on the Kidney in Male wistar Rats. Twenty (20) adult wistar rat (weighing 195g) were used for the study. The animals were randomly assigned into four group with five rats in each group. Group A was tag as control, while B, C, and D were labeled as test groups. Group A control was not given other thing but water and field. Group B was given chloroquine once a day for 7 days; animal in group C were given chloroquine once in a day for 14 days, while group D animals given chloroquine once in a day for 21 days. All the rats in group A (control) were sacrificed. All rats in the test (group B) were sacrificed after 7 days. All rats in the test (group C) were sacrificed after 14 days. All rats in the test (group D) were sacrificed after 21 days. The rats were sacrificed using chloroform. Blood samples were collected to determine urea, creatinine, sodium, potassium, chloride, bicarbonate and the kidney was harvested for histological examination. Data obtained was subjected to analysis using the Statistical Package for Social Sciences (SPSS), version 17.0. Results were presented as means ± standard deviations. One-way analysis of variance (ANOVA) was used for comparison of the means. Differences between means was considered to be significant when P < 0.05. From this study it was observed that chloroquine administration induces nephrotoxicity in male Wistar rats. This nephrotoxicity is characterized by histopathological changes, including tubular necrosis, interstitial inflammation, and glomerular injury.


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References


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