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Effect of Clomiphene Citrate on Some Reproductive Organs (Uterus, Vagina, Uterine Wall And Ovary) of Female Albino Rats With Regular Estrous Cycle
Abstract
The aim of this study is to show the effect of Clomiphene citrate on the Uterus, Vagina, Uterine wall, ovary and the histology of female wistar rats on regular estrous cycle. A total of thirty (30) 10 weeks old healthy female Wistar rats were purchased for the purpose of this study. Group 1(Control) were the animals in this group were not exposed to any experimental agent/drug. During the nine days of the trial, they had unrestricted access to food and clean water. Group 2's animals received 9 days straight of exposure to the test medication, clomiphene citrate 1 mg. For Group 3 the animals in this group were exposed to the test drug (Clomiphene citrate 2mg) for 9 consecutive days. In Group 4 the animals in this group were exposed to the test drug (Clomiphene citrate 5 mg) for 9 consecutive days.Vaginal smears were obtained from all rats to determine whether they had regular cycles for three cycles before drug administration. This study shows the body weight for regular estrous cycle at day 1, 3, 5, 7, 9 and 11 of control and test subjects in which of all the test groups none were found statistically significant (p<0.05) when compared with their respective control. In respect to the uterine wall, weight, there were non significant (p>0.05) changes in uterine wall, vagina and uterus weight from group B, group C and group D. Results of the present work showed that clomid induced many histological alterations in the ovary of rats in which Group B showed degenerating follicles, Group C showed enlarged graffian follicle, congested blood vessels and degenerated follicles while Group D showed enlarged graffian follicle, degenerated follicles and congested blood vessels. The histology of vagina for all test groups showed smooth inside layer, squamous epithelium and lamina propia. The histology of the uterus, shows normal histology with Myometrium, the endometrium and a small slit like lumen for control, For Group B, the histology of the uterus show simple columnar cells with basal oval nuclei. Group C Uterus histology show endometrial hyperplasia and pseudo-stratification and Group D uterus histology show stroma densely cellular (C), epithelial eosinophilic infiltrations with prominent eosinophilic brush borders of surface epithelial cells.
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