Role of Tissue Culture Technique on Wheatgrass (Triticum aestivum): A Staple Food Commonly known as Wheatgrass Beneficial for Human Health

Abstract

Wheatgrass is a type of worldwide acceptable staple food. The scientific name is Triticum aestivum belongs to Poaceae family. The plant is proved to be superfood which has all nutrients and antioxidants. Many Scientists and researchers reveals many positive experiment through tissue culture techniques on Wheatgrass. They are responsible to boost up immune system, aid in digestion, and provide energy. The color of plant is green in colour having healthy shoots and roots. It is regarded as a super potent health food with amazing benefits. In present work, the different types of plant growth regulators were taken. It includes Auxin, Gibberlin, Cytokinin and IBA. These hormones were taken in different concentration like 10^-3M, 10^-6M and 10^-9M, MS media was taken which was supplemented with different concentration of hormones. Here two types of media were used i.e liquid and solid media. The liquid media was supplemented with hormones and MS media, while in solid media both MS and 0.8% Nutrient Agar were used. In liquid media, seed culture was done in Ms media supplemented with hormones. Out of four types of Plant Growth Regulators, Auxin plays a good role in morphogenesis and growth of shoots and roots in both liquid and solid culture. The seed germination started within 48 hours of inoculation. Within one week of short interval of period, the shoots measures 7cm and the length of roots ranges upto 4cm in length. In solid supplemented media, the shoots and roots ranges upto 4cm to 5.2 cm in length in 10^-6M Auxin supplemented media. Later, both protocols cultured in solid and liquid media were transfer to sterilized pot provided with soil. The parameters of soil were checked before inoculation of plants in pots. In solid culture, the nutrient Agar arises good results in morphogenesis and growth of roots and shoots of plantlets. The concentration of 10^-3 M and 10^-9M of Gibberlin, Cytokinin and IAA supplemented media shown a very negligible result on growth and morphogenesis of plantlets. On the contrary to other concentration 10^-6 M plus MS media considered a good candidate for developing healthy plantlets. The nature of cultivated in vitro plants of wheatgrass resembles same as the natural plants in morphology but nutrient contents seems to be altered. The objective of this study was to evaluate the role of tissue culture technique on the fruitful production of protocols with the application of Plant Growth Regulators. The nutritional analysis was also done from the plants of Wheatgrass harvested from in vivo and in vitro plants.

Cite as:

Babita Kumari, & Sangeeta Pal. (2024). Role of Tissue Culture Technique on Wheatgrass (Triticum aestivum): A Staple Food Commonly known as Wheatgrass Beneficial for Human Health. Journal of Advances in Nanotechnology and Its Applications, 6(3), 4–12. 

https://doi.org/10.5281/zenodo.13283691